Title: Effect of Shenfu parenteral injection on Bcl-2,Bax and c-fos proteins expression in rat ischemia-reperfusion myocardium
Abstract: Objective To observe the relationship between acute ischemia-reperfusion injury and apoptosis related gene Bcl-2 and Bax and c-fos protein expression,and the intervention of Shenfu parenteral injection(SF) in rats.Methods The ischemia-reperfusion(IR) model was established by ligating the left anterior descending coronary artery.Thirty-five healthy rats were randomly divided into sham operation group(group C),IR group and SF group(8 ml/kg SF was intravenously injected 15 min before ligation).After myocardial ischemia lasting for 40 min,the myocardium was reperfused for 30 min or 120 min,then the expression of Bcl-2 and Bax and c-fos proteins was detected in myocardium by immunohistochemistry.Results As compared with group C,the protein expression of Bcl-2 and Bax and c-fos in the ischemic myocardium significantly increased(P0.01),and Bcl-2/Bax ratio significantly decreased(P0.01) in IR group.As compared with IR group,the protein expression of Bcl-2 significantly increased(P0.01),and Bax and c-fos significantly decreased(P0.01),Bcl-2/Bax ratio significantly increased(P0.01) in SF group.Conclusion Protective effect of SF on ischemia-reperfusion myocardium may concern with restraining myocardial cell apoptosis through promoting Bcl-2 protein expression and decreasing Bax and c-fos protein expression as well as increasing Bcl-2/Bax ratio.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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