Title: The effects of propofol anesthesia in neonatal period on hippocampal NeuN expression of adult rats
Abstract: Objective To observe the long-term effects of propofol anesthesia in neonatal rats on cognitive function and hippocampal NeuN expression when growed up.Methods One hundred male sprague dawley rats aged 7 d were randomly divided into 5 groups (n=20 each): control group (group C) and propofol groups which received intraperitoneal (IP) propofol 25, 50, 100 and 200 mg/kg respectively (groups P1, P2, P3, P4). Arterial blood samples were obtained immediately after the animals were fully recovere for blood gas analysis. The rats were feeded up until 9 weeks old and spatial learning and memory were tested by Morris water maze. The animals were then killed and their brains were removed for detection of NeuN expression in hippocampus by immunohistochemistry method and Western blot analysis.Results There was no significant difference in PaO2, PaCO2, SaO2, HCO-3,BE and pH among the 5 groups. Compared with groups℃and P1, the escape latency was longer in groups P2,P3 and P4 by dose-dependent way. The frequency of passing through the platform in groups P2,P3 and P4 was (4.23±1.08),(2.71±1.24) and (2.83±1.05), which was less than that in groups℃and P1. NeuN-positive neurons and NeuN protein were decreased in groups P1, P2,P3 and P4 by dose-dependent way.Conclusion Propofol may have long-term effects on the development of brains in neonatal rats. Propofol may impair spatial learning and memory function of adult rats by dose-dependent down-regulation of neurons and NeuN expression.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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