Title: Sequence analysis of Xa21 gene promoter in rice(Oryza sativa 'Nipponbare') and its expression characteristics under different conditions
Abstract: Taking cultivar'Nipponbare'of rice(Oryza sativa L.) as the experimental materials,Xa21 gene promoter was cloned and sequenced by a pair of primers designed on the basis of genomic DNA sequence of same cultivar published on GenBank.Xa21 gene promoter with sequence length of 1 982 bp is obtained by PCR amplification,in which,except with promoter basic elements,it also contains some elements related to stress signals,such as GCC-box,A-box,TC-rich repeats,MBS,LTR and W-box,etc.By means of GUS histochemical staining and quantitative assay methods,expression specificity of Xa21 gene promoter in different organs and developmental stages of T1 strain of transgenic rice and its expression characteristics under different conditions with stress and hormone treatments were researched.The results indicate that GUS activity in leaf,stem and root of transgenic rice can be detected but that is the highest in root,especially in stele of root tip.With increasing of seedling age(3-,5-and 8-to 9-leaf stages),GUS activity in leaf increases gradually and reaches the highest at 8-to 9-leaf stage.Treatments with mechanical damage or 100 μmol·L-1 methyl jasmonate(MeJA) can lead GUS activity to increase significantly or extremely significantly,respectively,but treatments with drought,500 μmol·L-1 salicylicacid(SA) or100 μmol·L-1abscisic acid(ABA) have no obvious effect onGUS activity. It is suggested that external stress has an induction to expression of Xa21 gene promoter in rice,and the expression of promoter is controlled by developmental stages and possesses the specificity in organ or tissue with the highest expression in root.Disease resistance response mediated by Xa21 gene promoter depends on the jasmonate acid(JA) signal pathway.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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