Title: Observation of the Effect of PAP on the Rat's Hepatic Microcirculation in Thermal Ischemia Reperfusion Injury Period
Abstract: Objective To investigate the effect of papaverine(PAP) on improving and protecing the hepatic microcirculation in therma1 ischemia reperfusion injury period(IPI) and porbing into the feasibi1ity of administering medicine by portal vein.Methods Twenty wistar rats were divided into two groups randomly, experimental group(PAP 3.18 mg/kg) and control group(NaCl 4ml); the model of orthotopic liver thermal ischemia reperfusion was duplicaed in misra method and then PAP and NaCl were projected respectively by portal vein catheterization after reperfusion. Converting the microscope to observe the number of dilated sinusoids, the speed of blood flowing and the change of terminal hepatic venules caliber before and after giving PAP and NaCl. After that, the sera levels of SOD?ALT?AST?LDH were measured and pathological examination of its liver was done.Results 1. After duplicating model, the diameter, the blood flowing speed of central hepatic venules, the number of dilated sinusoids hepatic venules were considerably decreased in ischemia reperfusion injury(P0.05, P0.05, P0.01,respectively), 2, After giving PAP, the diameter, the blood fiowing speed of central hepatic venules, the number of dilated sinusoids hepatic venules were significantly increased(P0.05,P0.01,P0.05,respectively).3,The level of SOD in sera in experimental group was higher than that in control group(P0.05),the level of ALT, AST and LDH in experimental group was lower than that in control group(P0.01); 4.The expansion of liver sinusitis was faster(P0.05) and hepatocelluar cloudy swelling degeneration and necrosis were less severe (P0.05) in experimental group than those in control group. Conclusion 1. There are severe microcirculatory malfunction and ischemia reperfusion injury in hepatic thermal ischemia reperfusion.2. PAP can protect microcirculation and improve reperfusion injury effectively.3.Administering medicine by portal vein has distinct effect on improving hepatic microcirculation in vivo ischemia reperfusion period.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot