Title: Role of toll-like receptor 4-dependent signaling pathway in lipopolysaccharide-induced human lung carcinoma cell proliferation
Abstract: Objective:Our aim was to study the mechanism through which toll-like receptor 4(TLR4)-dependent signaling pathway was involved in lipopolysaccharide(LPS)-induced the proliferation of human lung adenocarcinoma A549 cells.Methods:MTT assay and flow cytometry(FCM) were performed to examine the proliferation of A549 cells after stimulation with LPS.Immunocytochemistry staining was used to evaluate the TLR4,c-Jun,c-Fos and nuclear factor-kappa B(NF-κB)protein expressions in A549 cells after treatment with different concentrations of LPS.Results:LPS stimulated the proliferation of A549 cells,especially after 24 h treatment.The ratio of cells in G2/M phase in the 100 ng/mL LPS group was significantly higher than that in 10 ng/mL LPS group.The difference was significant(P0.05).The expressions of TLR4,c-Jun,c-Fos,and NF-κB detected in A549 cells were gradually elevated with the increase in the concentrations of LPS.The expressions of TLR4,c-Jun,c-Fos and NF-κB were the highest in LPS(100 ng/mL) group compared with the control group(P0.05).Conclusion:LPS promoted the proliferation of A549 cells.The mechanism may be explained as follows: binding with TLR4 receptor,stimulating TLR4 signaling pathways,and then activating AP-1(heterodimer of c-Jun and c-Fos) and NF-κB,finally inducing persisted proliferation of human lung carcinoma cells.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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