Title: Effects of shark hepatic stimulator substance on acute liver injury and liver mitochondrial function in rats
Abstract:Objective:To assess the effects of shark hepatic stimulator substance (sHSS) on thioacetamide (TAA)-induced acute liver injury and liver mitochondrial function in rats. Methods: Acute liver injury was...Objective:To assess the effects of shark hepatic stimulator substance (sHSS) on thioacetamide (TAA)-induced acute liver injury and liver mitochondrial function in rats. Methods: Acute liver injury was induced by 2 consecutive intraperitoneal injections of TAA (400 mg/kg) at 24-h intervals. Rats in therapy group were initially treated with sHSS (80 mg/kg/) 1 h prior to TAA;rats in control group were treated with the same volume of 0.9% sodium chloride. Twenty-four hours after the second injection of TAA, changes in serum ALT and AST activities, MDA level in the liver, and the effects of TAA and sHSS on liver mitochondrial respiratory function, swelling and membrane potential were investigated. Results: The levels of serum ALT, AST and MDA were significantly lower in therapy group compared to those in control group(P0.05). In therapy group, the ADP-stimulated oxygen consumption, the respiratory control ratio (RCR), the ADP/O value and the oxidative phosphorylation ratio in isolated mitochondria were significantly higher than those of control group(P0.05). The swelling and the change of membrane potential of liver mitochondria were not induced by the injections of TAA and sHSS. Conclusion: sHSS can obviously prevent acute liver injury and lipid peroxidation, and can improve mitochondrial respiratory function impaired by TAA.Read More
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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