Title: Effects of maternal lead exposure on the expression of phosphorylated tau in hippocampus of mouse offspring
Abstract:To explore the effects of maternal lead exposure on the expression of phosphorylated Tau protein and to reveal the underlying molecular mechanism of neurotoxicity induced by lead, lead exposure was co...To explore the effects of maternal lead exposure on the expression of phosphorylated Tau protein and to reveal the underlying molecular mechanism of neurotoxicity induced by lead, lead exposure was conducted through freely drinking the corresponding lead acetate solutions with dosages of 0.3 g · L-1, 1.0 g · L-1, and 3.0 g · L-1, respectively. Each group was composed of 10 mice. The lead contents in blood and hippocampus of the offspring 21 days after birth were determined. On the 21st day, the phosphorylation expression of Tau protein in hippocampus of all offspring in various dosage groups was determined by immunohistochemistry assay and the Western Blot. The lead levels in blood and hippocampus of 21d lead-treated pups were higher than those in the control group (p0.05). The results of immunohistochemistry showed that the P-tau was mainly located in the cytoplasm, The area density of P-tau positive immune reaction in CA1 area of mouse pups in three lead exposure groups were higher than the control group (p0.05), but the average gray value in lead exposure groups were lower than the control group (p 0.05). The results of Western Blot showed that the expression of P-tau protein in hippocampus of medium and high lead exposure group pups was higher than the control group (p0.05), but the expression P-tau between low lead exposure group and the control group had no statistical significance (p0.05). Maternal lead exposure may induce lead accumulation in hippocampus of offspring. Lead exposure during pregnancy enhanced the expression of P-tau protein in hippocampus of pups and subsequently affected the release of neurotransmitter, which may lead to the learning and memory damages.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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