Title: The protective effect of dexmedetomidine preconditioning and its effect on complement component 3 in rats with myocardial ischemia reperfusion injury
Abstract: Objective: To investigate the protective effect and possible mechanism(effect on complement component 3)of dexmedetomidine preconditioning on the myocardium of ischemia and reperfused rats. Methods:Forty-five Sprague-Dawley male rats were divided into sham operation group(Sham group), ischemic-reperfusion group(I/R group) and dexmedetomidine preconditioning group(DEX group) randomly, with 15 rats in each group. Before left anterior descending coronary artery was clamped, DEX group received dexmedetomidine with 5 mL·kg-1 body weight for 2 h(dexmedetomidine was diluted to 1 μg·mL-1with 0.9% sodium chloride injection). While I/R group and Sham group were infused 0.9% sodium chloride injection with 5 mL·kg-1 body weight for 2 h. At the end of 120 min reperfusion, the myocardial tissues were taken for determination of the infarct size and C3 expression using the Western blot method. Results:Compared with the Sham group, the infarct sizes and expressions of C3 increased obviously in the I/R group and DEX group(P 0.05). Compared with the I/R group, the infarct sizes in DEX group were smaller and expressions of C3 were lowe(rP 0.05). Conclusion:Dexmedetomidine could attenuate myocardial ischemiareperfusion injury in rats via suppressing the expression of C3.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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