Title: Quality Control on Radix et Rhizoma Rhei in Qianliening Granule
Abstract: Objective To establish the HPLC method in the determination of emodin and chrysophanol in Qianliening granule.Methods The contents of emodin and chrysophanol were determined by Shimadzu LC-10A and the separation was performed on Shimadzu C18(4.6 mm×250 mm,5 μm) with mobile phase consisted of methanol-0.1% phosphoric acid solution(75:25) at a flow rate of 1.0 mL.min-1,and the detective wavelength was 254 nm.Results The linear range of emodin was 0.01803~0.9016 μg(r=0.99992),and chrysophanol was 0.01198~0.5992 μg(r=0.99993).The average recovery of emodin and chrysophanol were 98.04% and 97.74% respectively,and the RSD were 0.75% and 0.62% respectively.Conclusion The method shows good separation effect,accuracy,reproducibility and quick analytical speed,can be used for the quality control of Radix et Rhizoma Rhei in Qianliening Granule.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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