Title: Differential regulation of neurotrophic factor and it receptor mRNA and protein by chronic fluoxetine and stress in the adult rat hippocampus and Amygdaloid nuclei
Abstract: Objective To observe the effect of chronic unpredicted sequence of mild stress(CUMS)and fluoxetine on the expression of brain-derived neurotrophic factor(BDNF),neurotrophin-3(NT-3)and their functional receptors TrkB/trkC in the hippocampus and Amygdoid nuclei of adult rat.Methods Thirty-two male SD rats were randomly and equally allocated to 4 groups.Quantative reverse transcription polymerase chain reaction(RT-PCR)and immunohistochemistry were used to examine the expression levels of BDNF/TrkB and NT-3/TrkC protein and mRNA after CUMS and fluoxetine management.Results After the experiment,the opposite regulation of BDNF in the hippocampus and similar in the Amygdoid nuclei were shown to compare with their control groups,different expression direction of NT-3 in the hippocampus and Amygdoid nuclei was presented.And changes in the pattern of their receptors TrkB/trkC differed from their corresponding ligand.Conclusion Different models of expression in CUMS and fluoxetine groups suggested a role for them in the onset of antidepressive effectivity and the pathogenesis of depression.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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