Title: Development of Real-time PCR for Rapid Detection of Salmonellaspp in Animal-origined Food
Abstract: A real-time PCR was developed for the rapid detection of Salmonella spp based on the primers and probes which were designed for the conservative domain of fimY gene of Salmonellaspp.The sensitivity of the real-time PCR was 5.2cfu/mL.56 from 781samples of meat,eggs,milk aquatic products were positive in real-time PCR assay,which was in accordance with the testing result according to GB/T4789.4-2008.The results showed that a real-time PCR assay was sensitive,rapid and simple.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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