Title: Simultaneous Determination of Paeoniflorin and Paeonol in Paeonia suffruticosa by Modified RP-HPLC
Abstract: OBJECTIVE:To provide more effective means for the quality control of Paeonia suffruticosa by improving the method for simultaneous determination of paeoniflorin and paeonol in P.suffruticosa.METHODS:Agilent ZORBAX Eclipse XDB C18(250 mm×4.6 mm,5 μ m) column was used with mobile phase consisted of methanol-water(gradient elution) at the flow rate of 1.0 ml/min.The detection wavelength was 230 nm for paeoniflorin and 274 nm for paeonol.RESULTS:The linear range of paeoniflorin was 0.060 33-1.206 6 μg and that of paeonol was 0.200 4-4.008 0 μg(r=0.999 9).RSDs of precision test,reproducibility test and stability test were all lower than 2%.The average recoveries were 99.49% and 98.66% with RSDs of 2.0% and 1.8% respectively(n=6).CONCLUSION:The modified method removes phosphate corrosion for chromatographic column.The method is simple,rapid and accurate,and can be used for the qualitative analysis of paeoniflorin and paeonol in P.suffruticosa and its preparations.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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