Title: Immune response induced by DNA vaccine Co-encoding of classical swine fever virus E2 and GM-CSF in mice
Abstract: To study effect of GM-CSF on immune response of mice elicited by immunization with DNA vaccine encoding of classical swine fever virus E2,CSFVE2 and GM-CSF amplified by PCR.were inserted into pcDNA3.0 to construct:pcE2,pcE2-GF and pcGF.50 female mice were randomly divided into 5 groups,10 each.Group A,B were immunized with pcE2 and pcE2-GF and group B,C and D are controls,immunized with pcDNA3.0,pc GF and physiological saline respectively.At weeks 0,2,4,sera were collected before every immunization and 2 weeks after final immunization.Serum lgG were detected by ELISA.Spleen lymphocyte cells of mice were collected 2 weeks after final immunization.Proliferation activity of spleen lymphocytes was detected by MTT assay.Results showed that compared with control groups,level of IgG in sera increased,and proliferation activity of spleen lymphocytes were increased.FurthermoreI,gG level in sera and proliferation activity of spleen lymphocytes of group B are higher than that of group A.In conclusion,GM-CSF can increase the level of immune response in mice induced by immunization with DNA vaccine encoding of classical swine fever virus E2.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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