Title: Screening and identification and antagonism research of two biocontrol bacillus strains
Abstract: [Objective] This study aimed to screen biocontrol bacillus strains with broad-spectrum and highly efficient antagonistic activity against plant pathogen fungi,and further to study the antagonist function.[Methods] Present study used eight kinds of plant pathogenic fungi as target bacteria to screen superior bacillus strains from 9 candidate strains,the method of flat confrontation,antagonistic activity determination of fermentation filtrate were used.[Results] Two bacillus strains B06 and B07 were found with the characteristics of broad-spectrum and highly efficient antagonistic activity.B06 was more effective in inhibiting 8 pathogenic fungi than other strains,with R2/R1 ranging from 0.4 to 1.8,and the inhibition rate of sterile fermentation filtrate from 66.7% to 87.5%.B07 took second place in inhibiting 8 pathogenic fungi than other strains,with R2/R1 ranging from 0.23 to 1.21,and the inhibition rate of sterile fermentation filtrate from 55.56% to 81.25%.Bacillus strains B06 and B07 were both identified as Bacillus amyloliqueaciens with the methods of 16S rDNA sequence analysis.[Conclusion] The result indicates that bacillus strain had the ability to inhibit kinds of plant pathogen fungi.Screening of bacillus strains with broad-spectrum and highly efficient antagonistic activity has a great development and application value in agricultural biological control.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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