Title: The Radioenzymatic Assay of Matrix Metal loproteinase-1 Activity
Abstract: The radioenzymatic assay method for tissue collagenase, a metalloproteinase, activity in matrix was established in this study. The matrix collagenase is the most vital catabolic enzyme of collagen in tissue. It mainly acts on type- I,II,III matrix collagen and is also called matrix metalloproteinase-1 (MMPs-1). Our assay method for the matrix collagenase was as follows: After type- I collagen was prepared from calf skin and identified with HPLC, it was marked with 3H-acefic auhydride as the substrate. Then a series of assays were performed in animal experiments and huamn cases, which showed that the matrix collagenase (MMPs-1) activity assay is feasible and gives reliable results in clinical biochemistry study.
Publication Year: 1998
Publication Date: 1998-01-01
Language: en
Type: article
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