Title: Prevention of acute GVHD after xenogeneic (rat→mouse) bone marrow transplantation with interleukin-11
Abstract: The total body irradiated BALB/c mice were injected intravenously with 4×107 SD rat bone marrow cells plus 4×107 splenic cells to establish a xenogeneic bone marrow transplant GVHD model. IL,11 at adosage of 250 μg/kg was given subcutaneously twice daily from days -2 to +7after bone marrow transplantation(BMT), the control group just received injection of diluent only. The survival of animals was monitored daily and the GVHD clinical score measured weekly. Serum levels of TNF,α, IFN,γ and IL,4 were determined at day 5 after xenogeneic BMT. The splenic T cells of animials were stimulated in vitro with xeno,antigen at day 14, and the production of TNF,α, IFN,γ and IL,4 was measured by ELISA. It was found, in comparison with the control group, the peak period of developing GVHD was delayed with reduced severity of illness and prolongation of the survival time in the IL,11 treated group. The degree of proliferation of the splenic cells in the IL,11 treated group of mice was significantly lower than that of the control group, and the addition of the exogenous IL,11 could inhibit the proliferation of lymphocytes. The amount of IL,4 produced by the lymphocytes of the treated group was 3 times of the control group, but the animals of IFN,γ and TNF,α produced in the treated group were significantly reduced. The serum levels of these cytokines were consistent with the results in vitro. These results indicate that administration of IL,11 prevents the development of lethal graft,versus,host disease in the rat to mouse xenogeneic bone marrow transplantation model.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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