Title: Laboratory study of HIV-1 p24 by immuno-PCR
Abstract: Objective To establish an immuno-PCR detection method with the carrier of golden-magnetic particles for laboratory detection of HIV-1 p24 antigen.Methods Using mouse anti-p24 monoclonal antibody as the capture antibody coating golden-magnetic particles,using biotinylated goat anti-p24 polyclonal antibody as the detection antibody.The reporter DNA is a fragment of cinnamoyl coenzyme A reucing enzyme gene of Brassica napus L,and it was initially generated by PCR amplification using a biotinylated primer,and was bound with streptavidin to biotinylated polyclonal antibody.Human recombinant p24 antigen sandwiched by antibodies was detected by amplifying the reporter DNA using PCR.And then the detection conditions were optimized and the sensitivity was anlyzed.Results To reduce the effect of nonspecific amplification,the optimal concentration of streptavidin and DNA label were determined to be 0.1mg/ L and 10ng/ L,respectively.The detection limit of the immuno-PCR assay was 0.1ng/L,an approximately 1.5×104-fold higher compared with an enzyme-linked immunosorbent assay.Conclusion The immuno-PCR for detection HIV-1 p24 antigen is indicated to be a high sensitive detection method.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot