Title: Coxsackievirus B3-Induced Apoptosis in HeLa Cells
Abstract:Objective:To observe the dynamic process of apoptosis in HeLa cells induced by Coxsackievirus B3(CVB3).Methods: After infection with CVB3,HeLa cells were harvested at different time points,cytopathic ...Objective:To observe the dynamic process of apoptosis in HeLa cells induced by Coxsackievirus B3(CVB3).Methods: After infection with CVB3,HeLa cells were harvested at different time points,cytopathic effect and apoptosis were detected by contrast microscopy,electron microscopy,flow cytometry and terminal deoxynucleotidyl transferase mediated dUTP nickend labeling(TUNEL).Results: Apoptosis of HeLa cells occured in 4 hours after coculture with CVB3,(11.79%±0.962% vs.4.07%±0.665%,P0.05),which peaked at 36-48h post-infection.However,apoptosis was inhibited in HeLa cells at 1hour post-infection with CVB3(2.21%±0.448% vs.3.73%±0.637%,P0.05).Conclusions: CVB3 infection induces apoptosis in HeLa cells,and virus-associated apoptosis may play an important role in the pathogenesis of cytopathic effect.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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