Title: Comparative Study of the Transfection Efficiency of Macrophage by Different Methods
Abstract: Objective: To transfect siRNA into murine macrophage by different methods, and comparison of different transfection methods. Method: The cells were transfected by different methods with lipofectamine,Roche transfection reagent,electroporation and lentiviral vectors,The analysis of transfection efficiency of different methods by flow cytometer,levels of target mRNA and cell viability were determined. Result: The efficiency of Lentivirus-mediated siRNA transfection is the highest,up to 34. 75 ± 5. 30%,and the RNAi effect is best; Followed by Roche transfection reagent and electroporation,but electroporation cell viability lowest; Lipofectamine difficult to transfected,only 12. 17 ± 1. 53%,but cell viability of Lipofectamine was greatest. Conclusion: Through the comparison of the four kinds of murine macrophage transfection methods,the advantages and disadvantages of four kinds of transfection methods was acquired,and provides the basis for murine macrophage transfection.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot