Title: Karyotypes Analysis and 45S rDNA-FISH of the Tetraploid and Diploid in Carica papaya L.
Abstract: By using the techniques of karyotype analysis and fluorescence in situ hybridization with 45S rDNA as probe,the characteristics of karyotypes and 45S rDNA-FISH of tetraploid and diploid in Carica papaya L.were studied.The results showed that the karyotypes of tetraploid and diploid were similar,the lengths of their chromosomes were 2.35-4.76 μm as short chromosomes.They were composed of metacentric chromosomes and submetacentric chromosomes,their karyotypes were 2A,the karyotype formulas were 2n=2x=18=16m(2SAT)+2sm and 2n=4x=36=32m(4SAT)+4sm.Two signals were detected in the karyons and chromosomes of somatic cells of diploids,and four signals were detected in that of tetraploids.These signals were all located in the Nucleolar Organizer Region existed in the sixth pair of homologous chromosomes with satellites.According to the results,it is concluded that tetraploids are homologous tetraploids which came into being as the reduplication of chromosomes of somatic cells of diploids.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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