Title: The Role of Bradykinin in Protective Effect of Angiotensin(1-7) on Endothelial Cell in Human Umbilical Vein Cells
Abstract: Objective To investigate the potential role of bradykinin(BK) in the protective effects of angiotensin(1-7) on human umbilical vein endothelial cells (HUVECs) injury and apoptosis induced by angiotensin Ⅱ (Ang Ⅱ) in vitro culture. Methods Cultured HUVECs were randomly grouped as follows: control, Ang Ⅱ, Ang(1-7), Ang Ⅱ+Ang(1-7), HOE-140, and Ang Ⅱ+Ang(1-7)+HOE-140. Spectrophotometry and flow cytometry were used to evaluate lactate dehydrogenase (LDH) leakage and apoptosis percentage. Nitric oxide (NO) and endothelin-1(ET-1) levels were measured by colorimetry and radioimmunoassay. Expression of intercellular adhesion molecule-1(ICAM-1) in HUVEC membranes was determined by immunohistochemistry and image analysis. Results After incubating the HUVECs with 0.1 μmol/L Ang Ⅱ for 24 hours, LDH leakage (231.5±74.4 vs control:76.6±19.4 U/L, P0.01), ET-1 release (97.6±15.7 vs control:48.8±9.3 pg/mL, P0.01), expression of ICAM-1(0.070±0.001 vs control:0.041±0.003, P0.01), and apoptosis percentage (24.8%±0.6% vs control:1.4%±0.6%, P0.01) were significantly increased. These increases were inhibited by cocultured with Ang(1-7) 1 μmol/L. BK subtype 2 receptor antagonist HOE-140(1 μmol/L) attenuated the inhibiting effect of Ang(1-7) on the performance by Ang Ⅱ. Neither Ang(1-7) nor HOE-140 alone had any effect on HUVECs. Conclusion Ang(1-7) was shown to have protective role on HUVECs via inhibition of Ang Ⅱ induced injury and apoptosis. The protection effect may be partially mediated by bradykinin B_ 2 receptor.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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