Abstract:Objective To detect brucella use PCR assay in genus and species levels.Methods Three published pairs of primers were used to detect the gene fragments of brucella DNA extracts from bacteria.Results PC...Objective To detect brucella use PCR assay in genus and species levels.Methods Three published pairs of primers were used to detect the gene fragments of brucella DNA extracts from bacteria.Results PCR assay can identification brucella spp.,B.melitensis and B.abortus(bv1,2,4),B.neotomae.The ability of the assay for brucella spp.,B.melitensis and B.abortus(bv1,2,4),B.neotomae identification were 100%,87.5% and 92.0%.Conclusions The PCR assay can detect the gene fragments of brucella DNA extracts from bacteria.B.abortus(bv9) is different from other biotype of brucella in gene levels.③There are differences in gene type and traditional type.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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