Title: Effects of human thioredoxin on pneumocyte apoptosis and Bcl-2/Bax expression in rats with lung ischemia/reperfusion injury
Abstract: AIM:To explore the relationship between apoptosis in the lung tissues and lung ischemia/reperfusion injury,and to observe the effects of human thioredoxin (hTrx) on apoptosis in lung ischemia/reperfusion injury. METHODS:The single lung in situ ischemia/reperfusion animal model was used. Eighty four Wistar rats were randomly divided into control group (control),groups of ischemia for 1 h and reperfusion for different times (IR1h,IR3h,IR5h),and groups of IR + human thioredoxin treatment (IR1h + hTrx,IR3h + hTrx and IR5h + hTrx). Transmission electron microscope (TEM),terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and immunocytochemistry techniques were used to observe apoptosis,apoptosis signal-regulating kinase 1 (ASK1) and expression of Bcl-2 and Bax in various phases of lung ischemia/reperfusion. RESULTS:Cell apoptosis in lung tissues was significantly high,ASK1,Bcl -2 and Bax protein were upregulated in lung tissues of lung ischemia/reperfusion injury as compared to control (all P 0. 01). Compared to IR group,hTrx suppressed apoptosis as well as expression of ASK1 and Bax protein (P 0. 01),Bcl-2 protein and the ratio of Bcl-2 /Bax were upregulated in lung tissues (all P 0. 05 or P 0. 01). There was a significant correlation between the expression of ASK1,Bax protein and cell apoptosis (r = 0. 775,r = 0. 814,respectively; all P 0. 01). There was a negative correlation between cell apoptosis and Bcl-2 /Bax protein (r = -0. 275,P 0. 05). CONCLUSION:Initiating cell apoptosis by the activation of Bcl-2/Bax system in lung tissues may contribute to the pathogenesis of lung ischemia/reperfusion injury. The protective effects of hTrx include suppressing the expression of ASK1,down-regulating the ratio of Bcl-2/Bax and blocking apoptosis in lung tissues in lung ischemia/reperfusion injury.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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