Title: Optimization of Condition for Expression of Recombinant ChMIL18-HN in Pichia Pastoris
Abstract: To research the most optimization condition for expression of recombinant ChMIL18-HN in Pichia Pastoris,the P.pastoris X-33/pPICZαA-ChMIL18-HN was expressed in a small flask culture system.By studying the yield of target protein under different time of methanol inducing expression,different pH of medium,different inducing dose of methanol and different inducing temperature,the most appropriate expression conditions were determined.The results indicated that recombinant fusion protein was expressed to be the highest under pH6.5,methanol concentration 1.5%,culture-inducing temperature 30 ℃ for 156 hours.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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