Title: Asymmetry and polarity of nucleosomes in chicken erythrocyte chromatin.
Abstract: Research Article1 January 1989free access Asymmetry and polarity of nucleosomes in chicken erythrocyte chromatin. S. C. Satchwell S. C. Satchwell MRC Laboratory of Molecular Biology, Cambridge, UK. Search for more papers by this author A. A. Travers A. A. Travers MRC Laboratory of Molecular Biology, Cambridge, UK. Search for more papers by this author S. C. Satchwell S. C. Satchwell MRC Laboratory of Molecular Biology, Cambridge, UK. Search for more papers by this author A. A. Travers A. A. Travers MRC Laboratory of Molecular Biology, Cambridge, UK. Search for more papers by this author Author Information S. C. Satchwell1 and A. A. Travers1 1MRC Laboratory of Molecular Biology, Cambridge, UK. The EMBO Journal (1989)8:229-238https://doi.org/10.1002/j.1460-2075.1989.tb03368.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Nucleosome dimers containing, on average, a single molecule of histone H5 have been isolated from chicken erythrocyte nuclei and the associated DNA fragments cloned and sequenced. The average sequence organization of at least one of the two nucleosomes in the dimers is highly asymmetric and suggests that the torsional, as well as the axial, flexibility of DNA is a determinant of nucleosome positioning. On average the nucleosome dimer is a polar structure containing linker DNA of variable lengths. The sequences associated with H5 containing nucleosomes and core particles are sufficiently different to indicate that removal of histone H5 (or H1) from chromatin may result in the migration of the histone octamer and a consequent exposure of sites for regulatory proteins. Previous ArticleNext Article Volume 8Issue 11 January 1989In this issue RelatedDetailsLoading ...