Title: Study on“kidney corresponding with winter” by differential expression gene in testicle in seasons
Abstract:Objective To screen gene fragment differentially expressed in testicle in winter and summer. Method Differential display reverse transcription PCR (DDRT-PCR) was used to detect the total RNA in testic...Objective To screen gene fragment differentially expressed in testicle in winter and summer. Method Differential display reverse transcription PCR (DDRT-PCR) was used to detect the total RNA in testicle sample. Results ①Forty-four gene fragments differentially expressed were obtained. Ten gene fragments sequence were detected and 7 of them had higher homology with known genes. ②Three new genes were discovered and were included by gene bank (CK743226,CK743227,CK743228). Conclusion ①In physiological situation, seasonal variation of winter and summer can result in change of gene expression in testical. ②Conarium regulats gonad according to season by differential expression of gene. ③Three new genes may be needed by animals for self-regulation according to season and for evolvement . ④The theory of “kidney corresponding with winter” has substance basis. Its regulating mechanism is related to conarium’s regulation to differential expression of gene in testical.Read More
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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