Title: Purification and characterization of a recombinant chitosanase from Bacillus subtilis
Abstract:A chitosanase gene from thermophilic Bacillus subtilis WY34 was cloned and expressed in E.coli.The recombinant chitosanase was purified and characterized.The chitosanase gene consisted of 723 bp and e...A chitosanase gene from thermophilic Bacillus subtilis WY34 was cloned and expressed in E.coli.The recombinant chitosanase was purified and characterized.The chitosanase gene consisted of 723 bp and encodes a polypeptide of 240 amino acids residues.Homology analysis of gene sequences showed that the enzyme shared 98% identity with the chitosanase gene from Bacillus subtilis 168.Crude enzyme was purified to homogeneity by Ni-IDA affinity chromatography.The specific activity of the purified chitosanase was up to 1 051.8 U/mg.The optimal pH and temperature for the purified chitosanase were 6.0 and 45 ℃,respectively.The enzyme was stable below 40 ℃ and from pH 4.5 to 8.0.The chitosanase showed excellent hydrolysis ability towards chitosan and produced a mixture of chitobiose,chitotetraose and some chitooligosaccharides with a longer chain length.These results indicated that the enzyme was an endo-type chitosanase and might be a good candidate for biotechnological application to produce chitooligosaccharides.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot