Title: Berberine Increases the Expression of ATP-Binding Cassette Transporter A1 and Cholesterol Efflux in THP-1 Macrophages via Deacetylation of Liver X Receptor-α
Abstract:Aim To investigate the effect and mechanism of berberine on ATP-binding cassette transporter A1(ABCA1) expression and cholesterol efflux in THP-1 macrophage. Methods THP-1 macrophage foam cell were ex...Aim To investigate the effect and mechanism of berberine on ATP-binding cassette transporter A1(ABCA1) expression and cholesterol efflux in THP-1 macrophage. Methods THP-1 macrophage foam cell were exposed to berberine with different levels(0,5,10,20 μmol/L) for 24 h or treated with berberine(20μmol/L) for increasing periods of time(0,6,12,24 h).Western Blot was used to determine protein levels for ABCA1,liver X receptor-α(LXR-α) and acetylated LXR-α.Cellular lipid accumulation was determined by high performance liquid chromatography analysis.Cholesterol efflux was determined by liquid scintillator. Results Compared with control group,berberine increased the expression of ABCA1 in dose(0~20 μmol/L)and time(0~24 h)dependent manner while decreased the acetylation of LXR-α.In addition,berberine increased the levels of cholesterol efflux and decreased the accumulation of cholesterol in THP-1 macrophage foam cells when the content of the berberine was 20 μmol/L and treated time was 24 h,the blends had the maximum impact. Conclusion Berberine may upregulate ABCA1 expression and cholesterol efflux via deacetylation of LXR-α.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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