Title: Induced Expression in vitro of Goat Mammary Gland Epithelial Cell of Transgenic Human Lactoferrin
Abstract:[Objective] The aim was to explore the technical system of induced expression in vitro of goat mammary gland epithelial cell,and evaluate expression situation of mammary gland specific vector efficien...[Objective] The aim was to explore the technical system of induced expression in vitro of goat mammary gland epithelial cell,and evaluate expression situation of mammary gland specific vector efficiency and foreign protein at the cell level.[Method] Goat mammary gland epithelial cell by human lactoferrin gene transfection was carried out induction culture in vitro using DMEM/F12 culture medium with 5 mg/L insulin,5 mg/L prolactin and 1 mg/L hydrocortisone,and collected supernatant per 6 hours.Using SDS-PAGE and Western blotting to detect expression situation of foreign protein after supernatant concentrated.[Result] There was target protein expression in the induced culture medium,and molecular weight was about 42 KD.[Conclusion] Used induced expression method of goat mammary gland cell in the study can induce goat mammary gland epithelial cell to express foreign gene,it lays a foundation for researching heterologous expression of human lactoferrin gene and mammary gland bioreactor.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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