Title: Optimization of Liquid Fermentation & Culture Conditions of Aroma-producing Yeast-1
Abstract: The number of yeast cells was used as the main reference index and the effects of different carbon source,nitrogen source,pH and temperature on the number of yeast cells were studied.Based on single factor experiment,secondary polynomial regression equation model of four influencing factors had been constructed by response surface Box-Behnken design.The optimized culture mediums were glucose 10.52 %,peptone 5.06 %,KH2PO41 ‰ and MgSO4.7H2O 1 ‰,and the culture conditions were pH 6.28,and temperature at 28.76 ℃.Under the above conditions,the number of yeast cells reached up to 7.71×108.Verification test indicated that the experimental value was consistent to the forecast value.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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