Title: Determination of lymphocyte subsets in the peripheral blood of the patients with hand transplantation.
Abstract: Objective To investigate the changes in the activated lymphocyte populations in peripheral
blood of the patients with hand transplantation and utility of the dose adjustment of antithymocyte globulin (ATG) through CD3+ T cell count monitoring. Methods The lymphocytes from the posttransplant peripheral blood of the patients in different periods were immunological labeled by triple color fluoresecent monoclonal antibodies CD3 /CD4 / CD8 and dual color fluoresecent monoclonal antibodies CD3/CD(16+56), CD3 /HLA-DR; CD3、 CD?、CD5、CD3 / CD(16+56) and CD3 /HLA - DR were determined by using flow cytometry (FCM) . Results The levels of CD3+, CD4+ , CD8+ T - lymphocyte , the activated T - lymphocyte [ CD3+ /CD(1616 +56)+ , CD3+ /HLA - DR + ], the silent T -lymphocyte [CD3+ /CD(16+56)-, CD3+/HLA - DA- ] in the patients decreased significantly during the first post-transplant week and then increased gradually to the pretransplant level except of the level of CD8+ T ?lymphocyte which increased to the level higher. The posttransplant CD4/CD8 ratio decreased constantly ; B - lymphocyte (CD3 - / HLA -DR+) increased during the posttransplant 10 days and subsequently decrease gradually to the level slightly higher than the pretransplant level ; Nature kill cell (CDs' / CD(i6+56)*) increased significantly at the first post-transplant day and then decreased quickly and maintained the low level. Conclusion The daily dose of ATG (2mg/ kg/ d) just sufficient to maintain the level of CD3 + T cells at0.1xl0~(9)~0.4×l0~(9)/lis applicable. The dynamic determination of T-lymphocyte subsets is helpful to the diagnosis of rejection and survival in hand transplantation . The immunosuppressive agents have significantly effects on the T cell subsets in the patients.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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