Title: The Effect of Imiquimod Inhalation on TGF-β1,Smads in Mouse Asthma Models with Airway Remodeling
Abstract: Objective To observe the effects of aerosol Imiquimod on the airway remodeling and the expression of transforming growth factor-β1(TGF-β1),Smad2,and Smad7 in mouse asthma models.Methods Forty BALB/c mice were divided into 4 groups,ten in each group.Normal group(A), model group(B),Budesonide group(C),and Imiquimod group(D).Mice were sensitized on days 0 and 14 by OVA and challenged from days 24 to51 by OVA repeatedly to establish a chronic model of asthma characterized by airway remodeling.group D were interfered with aerosol Imiquimod 30 min before challenge,and group C were interfered with aerosol Budesonide 30 min before challenge.Group A were treated with saline instead of OVA.The outcome measurements included airway inflammatory indices,and the degree of mucus secretion in the lumens,the amount of collagen deposition around the bronchus,and depth of airway smooth muscle(ASM) by HE,PAS and Masson′s staining.The expressions of TGF-β1,TGF-β1mRNA,Smad2 mRNA,and Smad7 mRNA were detected by immunohistochemistry and RT-PCR. Results ① The airway wall,smooth muscle of group B were thicker than that of group A(P0.05),While these airway structural changes were decreased in group D and group C(P0.05);② mucus hypersecretion,goblet cell hyperpla-sia,airway smooth muscle hypertrophy,and RBM collagen deposition were obvious in group B(P0.05),while significantly decreased in group D and group C(P0.05);③ the expressions of TGF-β1,TGF-β1 mRNA and Smad2 mRNA in group B were significantly higher than those in group A(P0.05),while decreased in D and group C as compared with group B(P0.05);④ the expression of Smad7mRNA in C group was significantly higher than that in group B.Conclusion Imiquimod aerosol inhalation could decrease the expression of TGF-β1,TGF-β1 mRNA,Smad2 mRNA,increase the expression of Smad7 mRNA in chronic mouse asthma models and inhibit airway remodeling.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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