Title: Experimental studies on apoptosis of A375 cells of xenografted tumors induced by nanosecond pulsed electric field and its mechanism
Abstract: AIM:To investigate the effect of nanosecond steep pulse on the growth and apoptosis in A375 cells of xenografted tumors. METHODS: After establishment of 4 to 6 week-old female nude mouse models carrying human melanoma by subcutaneous inoculationg, 20 mice were randomly divided into 2 groups: Treatment group and control group. The mice were exposed to nanosecond steep pulse(20 kV/cm, 300 ns, 1 Hz in 5 min, three times every 2 d), the changes of the tumor body volume were observed and the inhibition rate was calculated. Caspase-3 was determinated by immunofluorescence and RT-PCR and the apoptosis of A375 cells was detected by transmission electron microscope. The changes of [Ca2+]i in A375 cells were observed by fluorescence spectrophotometer using Fluo-3/AM as the calcium fluorescent indicator. RESULTS: The tumor body volume in treatment group was smaller than that in control group(P0.01) and typical morphological features of apoptotic cells were observed under transmission electron microscope. Immunofluorescence and RT-PCR showed that caspase-3 in treatment group increased compared with that in control group. [Ca2+]i markedly increased by treatment with nanosecond steep pulse(P0.01). CONCLUSION: Nanosecond steep pulse induces apoptosis of transplanted tumors.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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