Title: Effect of Rb_1 on adult rat cardiomyocytes apoptosis
Abstract: Objective:To examine whether the apoptosis of adult cardiomyocytes could be induced by ischemia reperfusion,and to observe the effect of Rb 1 on the apoptosis.Methods:Agarose gel electrophoresis,TdT mediated dUTP nick end labeling (TUNEL) and fluorescence spectrophotometry were used.Results:The group of ischemia reperfusion showed characteristic DNA ladders in agarose gels;the percentage of positive cells in TUNEL and the DNA fragmentation observed with fluorospectrophotometer were higher than that of control.The group of Rb 1 showed that the percentage of apoptosis in TUNEL was obviously reduced,and the percentage of DNA freagmentation was also less than that of control.Conclusion:The apoptosis of adult cardiomyocytes could be induced by ischemia reperfusion injury and inhibited by Rb 1.
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
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