Title: Development of ELISA Method for Residue of Salbutamol
Abstract: An indirect competitive ELISA system to detect the residue of salbutamol was developed with sabutamol antibody and the performance about the detection system such as the sensitivity ,the detection limit ,the recoveries ,the precision and the specification were tested. And as the control method to this detection system,one High performance liquid chromatographic method (HPLC) to the residue of salbutamol was developed. The result showed the calibration curve of this ELISA detection system had the linear detection of 1~80ng/mL,the sensitivity of 0.58ng/mL and the detection limit of 1ng/mL. The recoveries of salbutamol in pork were 70~99%,and this ELISA detection system had respectively 107% cross-reactivity to clenbutarol and 10% to terbutalin,however,it had little cross-reactivity below 0.01% to Ractopamine and adrenaline. As the control detection systems,HPLC method had the linear detection of 10~200ug/mL,the sensitivity of 1ug/ml. The recoveries of salbutamol in pork were 80~95% through HPLC. In conclusion,compared with HPLC method,ELISA method had better sensitivity,specificity and approximative recoveries,but as estimated,ELISA method was not perfect enough in detec-tion result stability as HPLC.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 2
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