Title: Rapid Propagation and Sugar-free Tissue Culture for Male-sterile Line Cauliflower
Abstract: Used flower ball of Male-sterile line cauliflower as the explants for tissue culture and sugar-free technological research. The results showed that:when Induced 25 d differentiation rate of adventitious bud in the MS+BA1.0 mg/L+NAA 0.3 mg/L was 96%.The best medium of subculture was MS+BA 0.6 mg/L+NAA 0.1 mg/L. The proliferation rate was 4.25 times. The medium of sugar-free tissue culture was MS inorganic component +NAA 0.1. At light 4 000 lx and CO2 concentrations 1 000 mL/L, 11 d could be substantial root. Through this method, a flower ball, in the 4 months can produce more than 10 000 plantlets, this was a highly effective method.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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