Title: The expression of apoptosis-related factors-caspase-3 and caspase-9 in human clear cell renal cell carcinoma
Abstract: Objective To detect the proliferation,apoptosis and expression of apoptosis-related factors-Caspase-3 and Caspase-9 in human clear cell renal cell carcinoma(RCCC)tissues in order to explore the correlation between the two factors and RCCC.Methods The samples were divided into three groups:RCCC group,para-cancerous tissues group and normal tissue group.All the samples were fixed in formalin for 48 h,embedded in paraffin wax,sectioned to 5 μm thick slice,then observed by immunohistochemical method.Results The expression of caspase-3 and caspase-9 was weakly positive in normal renal tissue.The expression was increased in para-cancerous tissue.The expression was significantly decreased in RCCC tissue.There were significant differences among three groups(P0.01),but there were no significant differences among 4 stages(P0.05).Conclusion The expression of caspase-3 and caspase-9 protein in para-cancerous tissue is significantly higher than that in normal renal tissue,which is the lowest in RCCC tissue.The results suggest that the decrease of cell apoptosis and increase of cell proliferation is one of the important mechanisms in the tumor formation.There is no significant difference in the expression of caspase-3 and caspase-9 in different stages of renal clear cell carcinoma.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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