Title: Determination of total flavonoids in stemleaves of Chuanminshen violaceum by UV-Vis spectrophotometry
Abstract: Objective:To establish the determination method of the total flavonoids in stemleaves of Chuanminshen violaceum.Method:Reflux extracting method was used to extract the total flavonoids.The contents of total flavonoids were determined by UV-Vis spectrophotometry at wavelength of 506 nm with rutin as standard substance.Result:The linear range of rutin were 0.0212 mg﹒mL-1~0.0636 mg﹒mL-1(r=0.9992) with average recovery of 97.03%(RSD=2.3%,n=6).Conclusion:The method is accurate and simple,and it can be used for the content determination of total flavonoids in stemleaves of Chuanminshen violaceum.The research has laid a foundation for the utilization of Chuanminshen violaceum.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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