Title: 2D-PAGE Image Analysis of Proteins from Developing Embryos of Antheraea pernyi
Abstract: The present study analyzed variation of protein components and contents in Antheraea pernyi embryos of different developmental stages by SDS-PAGE and 2D-PAGE,so as to accumulate basic information on sequential expression of embryonic development genes in A.pernyi at the protein level.SDS-PAGE results showed that 32 major protein bands were isolated during the embryo development,including high abundance proteins with molecular weight of around 70,60 and 30 kD respectively.As the embryo development advanced,contents of these three proteins declined,while those of 40 and 15 kD proteins increased gradually.Proteins of 70 and 60 kD almost disappeared in newly-hatched larvae(324 h).Further analysis to 2D-PAGE images of embryonic proteins from different developmental stages showed that the protein spots increased gradually with embryo development.At 12 h of embryo development,there were 370 protein spots being detected.And at 300 h of embryo development,the number of protein spots reached 422.The matching rates of protein spots at 132 h and 276 h of embryo development with protein spots at 36 h of early embryo development was 52.4% and 28.4% respectively.More-over,2D-PAGE image of total proteins in newly-hatched larvae varied greatly with those at the embryonic stage.These results suggest that during the early stage of embryo development,i.e.from the embryo formation stage to appendage appearing stage(12~60 h),number of protein species were relatively low and did not change obviously;and after the trachea formation stage(228~276 h),protein species changed greatly,with increased number of acidic proteins and low matching rate of protein spots with previous stages.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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