Title: The effect of miRNA-197-3p-siRNA-1 entivirus on biological behaviors in human laryngeal carcinoma cell line Hep-2
Abstract: Objective To study the effect of lentivirus-mediated miRNA-197-3p-RNAi on biological behaviors in human laryngeal carcinoma cell line Hep-2. Methods miRNA-197-3p specific siRNA gene was synthesized and cloned into the recombinant lentiviral vector.Hep-2 cells were infected by miRNA-197-3p-siRNA(MI)recombinant lentivirus(miRNA-197-3p-siRNA-LV3).The Hep-2 cells were divided into MI group,negative control(NC)group and blank(B)group in vitro.The expression of the targets of miRNA-197-3p was detected by RT-PCR.Cell proliferation was detected by CCK8 kit.Cell cycle and apoptosis were detected by flow cytometry.The invasion was detected by Transwell test.Results The expression level of miRNA-197-3p was inhibited significantly by miRNA-197-3p-siRNALV3.The proliferation of Hep-2 was also markedly suppressed in CCK8(P 0.05).The number of invasive cells that migrated through the chamber decreased(P0.05).The cell cycle of Hep-2 and apoptosis had no obvious effect(P0.05). Conclusions miRNA-197-3p specific siRNA can suppress the proliferation and reduce the invasiveness of Hep-2 cells.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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