Title: Expression and significance of RANTES and CCR5 in chronic rejection of cardiac allografts
Abstract: Objective To investigate expressions of regulation upon activation of normal T-cell expressed and secreted(RANTES) and CCR5 in the chronic rejection of cardiac allografts.Methods Cervical cardiac transplantation by cuff-technique was performed from Wistar rats to Sprague-Dawley(SD) rats.The SD rat recipients were randomly divided into 3 groups:(A)(n=12) Both the recipients and the donors were without any treatment.(B)(n=12) The recipients were treated with 10 mg/kg-1 cyclosporine A after transplantation and euthanized 2 to 3 months later.(C)(n=24) The Sprague-Dawley rat recipients were pretreated with donor splenocyte(SPC) and cyclophosphamide(CP).Half were euthanized 15-60 days after transplantation(C1) and the other half were monitored for at least 6 months after transplantation(C2).Pathological change of the allograft caused by the rejection was observed by H-E staining.Cardiac allograft vasculopathy(CAV)was observed by Van Gieson staining.Expressions of RANTES and CCR5 were monitored by immunohistochemistry.Results Pretreatment of animals with SPC and CP induced long-term cardiac allograft survival.Immunohistochemical staining demonstrated a low level of RANTES and CCR5 expressions in the cardiac allograft muscles and coronary arteries in Group C.In contrast,expressions of RANTES and CCR5 in the cardiac allografts of Groups A and B were significantly higher than those in Group C(P0.05).The CAV and myocardial fibrosis were dramatically reduced in Group C compared with those of Groups A and B(P0.05).Conclusion Expressions of RANTES and CCR5 in cardiac allografts may play an important role during the development of chronic rejection.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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