Title: Construction and identification of recombinant retrovirus vector containg hTrx gene
Abstract: AIM: To construct a recombinant retrovirus vector carrying hTrx gene. METHODS: hTrx gene was cloned directionally into a retroviral vector pLXSN by DNA recombinant technique and the recombinant vector was identified Eco RⅠ and Bam HⅠ digestion analysis. The recombinant was transfected into packaging cells PA317 via CaPO 4 based transfection method, and viral titer in culture supernatant of transfected cellswas detected. RESULTS: The recombinant pLTrxSN had been constracted. Eco RⅠ and Bam HⅠ digestion analysis displayed two positive bands of 0.3 kb and 5.9 kb respectively. The viral titer in supernatant of transfected cells was 5.5×10 9 cfu/L. CONCLUSION: pLhTrxSN has been constructed successfully, which will be useful for Trx gene therapy and experiment study.
Publication Year: 2003
Publication Date: 2003-05-01
Language: en
Type: article
Indexed In: ['pubmed']
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