Title: Optimum fermentation medium of high-yielding neutral protease of mucor
Abstract: Actinomucor elegans and Wutungkiao Mucor were used as experimental materials,through single factor and orthogonal experiments,the optimization of fermentation medium composition for high-yielding protease of two kinds of hair mould was researched. The results showed that the optimal fermentation medium composition for neutral protease of Actinomucor elegans was:sucrose content 10%,soybean flour 2.5%,KH2PO40.4%,CaCl20.11%,the neutral protease activity was 1310.56U/mL. The optimal fermentation medium composition for neutral protease of Wutungkiao Mucor was:sucrose content 10%,soybean flour 3.0%,KH2PO40.37%,CaCl20.07%,the last neutral protease activity was 1306.38U/mL. The improve percentage of neutral protease activities were 60.1% and 46.7% respectively. This research laid the foundation for follow-up study of shortenning the fermentation cycle of fermented bean curd and production process of direct-loading fermented bean curd.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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