Title: Factors Influencing Agrobacterium tumefaciens-mediated Transformation in Trichoderma harzianum
Abstract: Agrobacterium tumefaciens-mediated transformation system was used to study on factors influencing transformation in Trichoderma harzianum. The results showed that the transformation efficiency was correlated with A. tumefaciens strains, bacterial cell volume initially used, concentration of conidia, co-cultivation time and acetosyringone induction. These findings should facilitate future study of T.harzianum and stimulate wider use of this valuable transformation method in other fungal transformation.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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