Title: Stable expression of antibacterial peptide CecropinB in dairy goat mammary gland epithelial cells.
Abstract: The paper aimed at constructing the eukaryotic expression vector of CecropinB and transtecting it into the goat mammary epithelial cell line. The recombinant plasmid inserted with CecropinB was transfected into the goat mammary epithelial cell by liposome Tfx(superscript M)-20. By screening with 0418, the stable transfected goat mammary epithelial cell line was established and the transcription and expression of CecropinB were identified by RT-PCR and agarose diffusion experiment, respectively. Results showed that eukaryotic expression vector pECFP-B was constructed successfully. Stable transfected goat mammary epithelial cell line was established and the CecropinB protein was expressed successfully. It provides the solid foundation for further experimental studies on the function of CecropinR
Publication Year: 2010
Publication Date: 2010-03-25
Language: en
Type: article
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Cited By Count: 1
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