Title: Cloning and expression of gene encoding PH domains of signaling molecules GRF
Abstract:Rat spleens were employed to clone the gene encoding PH domain of GRF. Total RNA molecules were isolated from fresh spleens and mRNAs were reversely transcriped into cDNAs. After amplification by PCR,...Rat spleens were employed to clone the gene encoding PH domain of GRF. Total RNA molecules were isolated from fresh spleens and mRNAs were reversely transcriped into cDNAs. After amplification by PCR, the fragments of DNA were cloned into vector pUC19. The method of dideoxy-termination was employed to sequence with labeled primmers, and then the gene was cloned into vector pGEX-4T-1 to express in fusion with GST. The sequence of the gene was correct and the gene was successfully expressed in pGEX-4T-1.Read More
Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
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