Title: Study of ERK5 regulating osteoblast function
Abstract: Objective To study the expression of ERK5 on human,rat,mouse osteoblasts and the regulation of IL-6 on ERK5 expression,preliminarily investigate the effect of ERK5 on proliferation and differentiation of osteoblast.Methods The protein expression of ERK5 on osteoblasts was confirmed by western blot.ERK5 and P-ERK5 were detected when different dose of IL-6 treated osteoblast during different time.ERK5 siRNA plasmids were constructed and transfected into MG-63 cells.MTT,ALP activity and osteocalcin expression were detected after treated with 10 nmol/L IL-6.Results ERK5 was expressed in human,rat,mouse osteoblasts.The phosphorylation level of ERK5 were increased by IL-6 on time and dose dependent manner.As compared to control cells,ERK5 siRNA cells' MTT reduced,osteocalcin expression reduced(P0.05) yet the ALP activity was no significant difference.Conclusion ERK5 signal pathway is regulated by IL-6 in osteoblast,and ERK5 signal pathway participate in the proliferation and differentiation of osteoblast.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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