Title: Effect of ischemic preconditioning on tumor necrosis factor-α interleukin-8 and interleukin-10 in Spraque-Dawley rats following myocardial ischemia and reperfusion
Abstract: AIM To observe the effects of ischemic preconditioning on the production of tumor necrosis factor-α(TNF-α),interleukin-8(IL-8) and interleukin-10(IL-10) following myocardial ischemia and reperfusion and to investigate its mechanism.METHODS Thirty-six Spraque-Dawley rats were randomly divided into three groups: ischemic preconditioning group(group IPC,n=12) was subjected to a 5 min occlusion of left anterior descending coronary artery(LAD),followed by reperfusion for 5 min four times and then elicited by 1h occlusion and 2 h reperfusion;ischemia reperfusion group(group IRI,n=12) was observed for 40 min and then elicited by 1 h occlusion and 2 h reperfusion;sham-operated group(group SO,n=12) was only observed for 40 min.At the end of the experiment,the right atrium myocardial blood and myocardial tissue were obtained and the serum TNF-α,IL-8 mass and myocardial tissue concentrations of IL-10 were detected.RESULTS In group IRI,the serum levels of TNF-α and IL-8 were(1 009±277) and(334±187) ng/L and inn group IPC,the serum levels of TNF-α and IL-8 were(778±178) and(229± 90) ng/L,significantly lower than those in IRI group(P0.05).In group IRI,the myocardial IL-10 was(6.8±2.6) ng/g and in group IPC,the myocardial IL-10 was(9.4±4.1) ng/g,significantly higher than those in IRI group(P0.05).CONCLUSION Ischemic preconditioning not only reduces the myocardial ischemia-reperfusion pathological process of proinflammatory cytokines TNF-α and the IL-8 produce,but also promotes the anti-inflammatory cytokines IL-10,suggesting that the protective effect of ischemic preconditioning on myocardial reperfusion injury through anti-inflammatory mechanism.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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