Title: Apoptosis of mouse peripheral lymphocytes induced by whole body γ irradiation and its mechanism
Abstract: Using in situ terminal labeling(ISEL), DNA electrophoresis and immunohistochemical methods, we studied the apoptosis and its mechanism of mouse peripheral lymphocytes induced by 2, 4, 6 and 8 Gy whole body γ irradiation and the expression of bax and bcl 2 gene products related to apoptosis was also studied. It was found that, ① At the early stage after irradiation (4 h ̄7 d) the percentage of lymphocyte apoptosis increased rapidly, for instance, 4 h after 2, 4, 6 and 8 Gy γ irradiation the apoptotic lymphocytes were as 2.6, 3.8, 5.5 and 10.4 times as that of control values, showing a good relationship between apoptosis and radiation doses; ② At the same time, with the increase in radiation doses the absolute counts of peripheral lymphocyte decreased sharply, for instance, 4 h after 2, 4, 6 and 8 Gy γ-irradiation the absolute counts of lymphocytes were 82%, 63%, 47% and 22% when compared with that of control values; ③ Using in situ terminal labeling method the peak value of lymphocyte apoptosis was observed on the 7th day after irradiation, and apoptotic lymphocytes were as 16 times as that of control value, which were in accordance with that obtained by May Grunwald Giemsa staining method. Meanwhile, the absolute counts of peripheral lymphocytes dropped to the lowest value on the 7th day after irradiation, suggesting that lymphocyte apoptosis might be major cause of lymphocyte reduction at the early stage after irradiation. ④ Abnormal expression of bcl 2 and bax gene products in irradiated lymphocytes indicated that they were closely related to the apoptosis in peripheral lymphocytes.
Publication Year: 1998
Publication Date: 1998-01-01
Language: en
Type: article
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