Title: Inhibition Effect of Corbicula fluminea Extract on Human Hepatocarcinoma SMMC-7721 Cells
Abstract:We obtained ethyl acetate extract(CFE) of Corbicula fluminea,and then investigated the antitumor activity of CFE.The inhibition rate of cancer cell was measured by MTT method.The morphological change ...We obtained ethyl acetate extract(CFE) of Corbicula fluminea,and then investigated the antitumor activity of CFE.The inhibition rate of cancer cell was measured by MTT method.The morphological change of SMMC-7721 cells was detected by inverted microscopy.The effects of CFE on cell cycles of SMMC-7721 were observed by FCM.The results showed that CFE displayed strong growth inhibitory effect in a dose and time dependant manner againsted hepatocarcinoma SMMC-7721 cells with estimated IC50 values of 70 μg/mL after 72 h of treatment with CFE.The morphological change of SMMC-7721 was significant.In the mean time,flow cytometry analysis showed that CFE could induce the emergence of the peak of apoptosis.These results revealed that CFE inhibited the proliferation and induced the apoptosis of the human hepatocarcinoma SMMC-7721 cells in vitro.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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